• Identification of oxidized lipids by GC/MS 
• Quantitation of prostaglandins & thromboxanes by isotope dilution GC/MS 
• Molecular weight determination of proteins & peptides by MALDI/TOF analysis 
• Microsequencing of proteins and peptides by capillary ESI/LC/MS & MALDI/TOF 
• Determination of postranslational modifications of proteins & peptides by ESI/LC/MS & MALDI/TOF 
• Quantification of micronutrients in physiologic fluids by GC/MS and ESI/LC/MS 
• Development of novel analytical methods using mass spectrometry 
• Interpretation & analysis of mass spectrometry data 
• Education & training in mass spectrometry principles & operation

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• high-quality cDNA, oligo, and Affymetrix microarrays for gene expression analysis from human, mouse, or rat samples. 
• extraction of RNA, labeling and hybridization of cDNA targets to microarrays, data capture, and archiving of microarray data. RNA quality assessment using an Agilent Bioanalyzer. 
• aid to investigators in the analysis and interpretation of microarray data. 
• ustom microarrays from unique clone and oligo sets developed for DDRC researchers. 

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• • Novel Cell Line Development Subcore
    • Utilizes the Immortomouse as a source of a conditional immortalizing gene in crosses with other transgenic mouse models that are relevant to a particular digestive disease. 
    • Establishes conditionally-immortalized epithelial cell lines from gastrointestinal mucosa, pancreas or liver.
    • Fully characterizes these cell lines with respect to their epithelial phenotype, the presence of the specific genetic mutation of interest and the presence of tissue-specific markers. 
    • Has established a “bank” of early passage cells for use by DDRC investigators.
  • Tissue Morphology Subcore
    • Assistance in procurement of human and animal tissues. 
    • Mechanisms for histopathologic characterization and quality assurance of specimens, database management, and specimen distribution. 
    • Centralization of histologic services under the supervision of a surgical pathologist with a dedicated interest in human gastrointestinal pathology and rodent models of gastrointestinal disease. 
    • Expertise in correlation of animal models with human disease, tissue characterization, immunohistochemistry, and laser microdissection. 

Customized tissue array block construction.

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• Access to and technical support for confocal laser scanning microscopy for studies on cell lines, polarized cells and tissues from the digestive tract.
• Support and UNIX based workstations with graphics systems for the display and analysis of massive data sets required for the images obtained.

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• Training, acquisition and analysis on our state-of-the-art cytometers for experiments that include up to 17 different fluorochromes simultaneously
• High-speed sorting with up to nine different fluorochromes simultaneously with the ability to sort and or clone into any plate or dish
• Soluble protein measurement from a variety of biological or experimental samples that can be multiplexed up to 30 different analytes concurrently
• Blood processing and storage to separate and collect cells, serum or plasma for subsequent studies
• Staining for subsequent flow cytometric acquisition and analysis of patient samples
• Expert consultation and troubleshooting and experimental design guidance

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