Transgenic zebrafish using transposable elements.

Publication Type:

Journal Article

Source:

Methods in cell biology, Volume 104, p.137-49 (2011)

Keywords:

Animalsdigestive disease, digestive deseases Animals, Genetically Modifieddigestive disease, digestive deseases Cloning, Moleculardigestive disease, digestive deseases DNA Transposable Elementsdigestive disease, digestive deseases Genetic Testingdigestive disease, digestive deseases Genetic Vectorsdigestive disease, digestive deseases Germ-Line Mutationdigestive disease, digestive deseases Microinjectionsdigestive disease, digestive deseases Mutagenesis, Insertionaldigestive disease, digestive deseases Zebrafish

Abstract:

DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.