Matrix-assisted laser desorption ionization imaging mass spectrometry: in situ molecular mapping.

Publication Type:

Journal Article


Biochemistry, Volume 52, Issue 22, p.3818-28 (2013)


Animalsdigestive disease, digestive deseases Breast Neoplasmsdigestive disease, digestive deseases Embryo, Mammaliandigestive disease, digestive deseases Fixativesdigestive disease, digestive deseases Lungdigestive disease, digestive deseases Micedigestive disease, digestive deseases Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationdigestive disease, digestive deseases Tissue Array Analysis


Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the tissue that are subsequently recorded in a mass spectrometer. IMS is distinguished by the ability to directly measure molecules in situ ranging from small metabolites to proteins, reporting hundreds to thousands of expression patterns from a single imaging experiment. This article reviews recent advances in IMS technology, applications, and experimental strategies that allow it to significantly aid in the discovery and understanding of molecular processes in biological and clinical samples.