Isolation of human eosinophils.

Publication Type:

Journal Article

Source:

Current protocols in immunology / edited by John E. Coligan ... [et al.], Volume Chapter 7, p.Unit7.31 (2012)

Abstract:

Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and differentiation from other leukocyte populations. Curr. Protoc. Immunol. 98:7.31.1-7.31.8. © 2012 by John Wiley & Sons, Inc.