Center for Molecular Studies in Digestive and Liver Diseases

Core Facilties

The Center is composed of four cores:


CELL CULTURE CORE

The Cell Culture Core maintains a centralized repository of cells and reagents pertinent to digestive, liver and pancreatic disease research. It also provides training (especially for students and postdoc fellows) for labs in new cell culture (2D and 3D) techniques.

Cells lines are established from freshly obtained surgical specimens, all aspects of which are approved by the University of Pennsylvania Institutional Review Board. Adenoviral, retroviral and lentiviral constructs are created, propagated and maintained under institutional guidelines for biohazardous materials. Provided is a listing of items currently carried by the Cell Culture Core:

  • Lists of available GI cancers and engineered human and mouse esophageal epithelial cells
  • Protocol for human esophageal epithelial cell culture
  • Protocol for mouse esophageal epithelial cell culture
  • Protocol for soybean trypsin inhibitor preparation used for mouse and human esophageal cell culture
Services
  • Normal human cell lines
  • Malignant cell lines
  • Training in 2D and 3D cell culture techniques
  • Training in design and use of adenoviral, retroviral, and lentiviral vectorsM


Visit the Core