Texas Medical Center Digestive Diseases Center

Core Facilities


CELLULAR AND MOLECULAR MORPHOLOGY CORE

The overall objectives of the Cellular and Molecular Morphology Core Laboratory are to provide expertise, equipment and procedures relevant to GI research, to make available its specialized laboratory facilities to GI researchers, and to develop new tests that may be useful in clinical and basic research.

This core is utilized by many members of the Texas Medical Center Digestive Diseases Center, and particularly serves pilot and feasibility awardees, whose experience and resources are necessarily limited. Major Core services include histology, immunohistochemistry, RNA in situ hybridization, mRNA probe generation, frozen sections for enzyme histochemistry, immunofluorescent antibody studies, live and fixed cell confocal, deconvolution microscopy and super resolution microscopy (SIM and STORM), and transmission electron microscopy, quantitative morphometric analysis, high throughput microscopy and high content analysis, laser capture microdissection for molecular genetic analyses, and digital images for internet communication and publication.

The core also provides consultation and training in the proper collection, fixation, storage and handling of human and animal tissues, as well as training and technical advice to researchers interested in developing sophisticated procedures for their GI-related research projects.

Services
  • Routine Histology & tissue processing with unstained slides furnished to investigator
  • Special stains, per slide (i.e. PAS, silver, trichrome)
  • Frozen section, preparation & furnished to investigator
  • Enzyme histochemistry (frozen sections or cells each slide)
  • Immunofluorescence (frozen sections or cells)
  • Immunohistochemistry
  • In-situ hybridization preparation and/or consultation
  • Electron microscopy, use and transmission EM including photographs
  • Immunoelectron microscopy
  • Scanning electron microscopy; sample prep (critical point drying)
  • Laser capture dissection
  • Quantitative morphometric analysis
  • Training in proper collection, fixation, storage and handling of human and animal tissues.
  • Consultation and technical advice to researchers interested in developing sophisticated procedures for their GI-related projects.

Visit the Core