Stanford Digestive Disease Center

Core Facilities

The Center consists of five enabling technology cores and an Administrative Core that manages the Pilot/Feasibility and Named Investigator Program, the annual Symposia and Retreats, the Enrichment Program (Seminars, Visiting Professors and Lecturers), and the Clinical Component.
The enabling technology cores are:


FACS/ IMMUNOASSAY CORE

FACS/Immunoassay Core - offering an array of services that allow study of single cells, flow cytometry, cell sorting, luminex and other services of the Human Immune Monitoring subcore

Services
  • Administrative/Clinical Component
    • Support and training for the design and analysis of clinical trials
    • Access to institution-wide patient care databases: STRIDE program
    • Facilitating target discovery and validation in humans: Tissue Microarray
  • Functions and Activities of the Flow Cytometry Component
    • Assistance and training in appropriate experimental design for flow cytometry and fluorescence-activated cell sorting experiments
    • Training and special immunofluorescence staining procedures for flow cytometry, including, but not limited to studies for up to 14 colors, apoptosis, DNA cell cycle analysis, bead assays, and studies examining kinetic changes
    • Fully assisted bulk and single cell sorting - FACS ARIA II and FACS Aria III
    • Fully assisted analysis - FACScan, FACScaliber, LSRII, Fortessa
    • Facility maintenance and troubleshooting
    • Training in flow cytometry applications and in individual use of the benchtop cytometers. Training for independent sorting on the two Arias
    • Independent, unassisted use of the bechtop cytometers and sorters by trained researchers during regular hours and on evenings and weekends
    • Subsidized access to FACS data analysis software (FlowJo) through a Site License. The Shared facility maintains a site license for use of FlowJo data analysis software. Facility users obtain FlowJo at a discounted rate of $195 per computer per year. DDC investigators using the Shared facility will have the option of using a DDC subsidy of 50% of the license fee to participate in the site license, and to download FlowJo onto their own computers, significantly facilitating analysis
  • Functions and Activities of the Immunoassay Component
    • Discounted and professionally run Luminex Bead Arrays, which provide the means to simultaneously assay up to 26 mouse or 51 different human cytokines in 100 ul samples
    • The NanoPro System for Signaling Analysis of Tissue and Cell Samples (Cell Biosciences) is an automated, rapid and quantitative protein measurement apparatus that combines nanofluidic isoelectric focusing with antibody detection to measure specific protein amounts and degrees of post translational modification, such as phosphorylation
    • The new CyTOF (for Cytometry by Time Of Flight mass spectrometry) flow cytometry system, allows simultaneous analysis of 30 or more analytes per cell. CyTOF mass spectrometry is a highly innovative multiparameter technology to study cell subsets and cellular responses
    • Other standardized assays performed by the HIMC include standardized flow cytometry immunophenotyping and phosphoepitope staining, gene expression and miRNA arrays

Visit the Core