Michigan Gastrointestinal Peptide Research Center


Core Facilties


MOLECULAR BIOLOGY CORE

The purpose of the Molecular Biology core is to facilitate the application of the tools of molecular genetics to the study of gastrointestinal peptides and their physiological functions. In all aspects, the Molecular Biology Core functions to provide essential services and serves as an educational resource to all Center Investigators. Although basic molecular techniques are widely available to Center investigators through commercial sources, there remains specific services that are best provided by pooled resources and by highly trained personnel such as those that can be provided by a Center Core facility. During the prior funding period, four Core programs were provided: the Transgenic Rodent Program, the Microarray Program, Viral Vector Program and the Molecular Techniques Program. The Microarray Gene Chip Program was added during the last competitive renewal and the Molecular Techniques Program was made available during the current funding cycle to provide education and training in those techniques that can be performed in the investigators own laboratory after receiving some theoretical and practical training. The latter is freely available as an educational resource for Center members interested in such techniques as chromatin structure analysis. Investigators can also obtain assistance with basic molecular techniques like quantitative RT-PCR as well as more sophisticated techniques like chromatin immunoprecipitation assays (ChIP) and ChIP-Seq.

Services
  • Transgenic Mouse Program
    • Consultation and Education
    • Transgenic Mice and Rats
    • Gene Targeting in ES Cells
    • Production of ES Cell Chimeric Mice
  • Viral Vector Program
    • Adenoviral Services
    • Retroviral Services
    • Adeno-associated Viral Services
    • Plasmid DNA Construction and Preparation Services
    • Real-Time PCR Services
    • Consultation
  • Microarray Gene Chip Program
    • Affymetrix Gene Profiling Services
    • Agilent BioAnalyzer Services
    • ABI 7900HT Quantitative PCR and SNP Genotyping Services
    • Biostatistical and Bioinformatics Support
  • Molecular Techniques Training Program
    • Education
    • Training
    • Review of Microarray Project Applications
    • Luminometer

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