Mayo Clinic Center for Cell Signaling in Gastroenterology (C-SiG)

Core Facilities

The Center is composed of three cores.


The Optical Microscopy Core plays an essential role in supporting the overall goals of Center members through three linked objectives:

Objective 1:To provide reliable, accessible, and state-of-the-art microscopic technology to all Center members that facilitates their study of GI cellular signaling cascades.

Objective 2:To educate and train Center members in the use of both basic and sophisticated cellular imaging methods. Emphasis is placed on providing technical instruction as well as educating faculty about how such approaches can expand the scope and breadth of their scientific programs.

Objective 3:To develop and apply state-of-the-art optical imaging technologies to GI tissues/cells.

  • Consults and training
    • 2-Photon microscopy
    • Coherent anti-Stokes Raman scattering (CARS) microscopy
    • 2-Photon microscopy
    • High-resolution, real-time imaging of live cells
    • Confocal microscopy
    • Confocal microscopy coupled with computer-based 3-D image reconstruction
    • Fluorescence resonance energy transfer (FRET)
    • Fluorescence recovery after photobleaching (FRAP)
    • Fluorescence loss in photobleaching (FLIP)
    • Expression and use of fluorescence-based bioprobes
    • Cellular microinjection
    • Total internal reflection fluorescence (TIRF)
    • Cell/tissue computer morphometry
    • Imaging cells in 3-D matrix
    • Data interpretation
    • Gel degradation assay
  • Reagents
    • Antibodies
    • Constructs
    • Cell lines

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