Mayo Clinic Center for Cell Signaling in Gastroenterology (C-SiG)

Core Facilities

The Center is composed of three cores.


GENETICS AND MODEL SYSTEMS CORE

Genetic manipulation of cells and model organisms is a critical scientific approach from biological modeling to establishing and testing critical molecular signaling pathways in both normal and clinically relevant disease states. Genetic tools are extremely dynamic, with new technologies becoming available every year. The C-SiG Genetics and Model Systems Core serves as a developer and provider of, current, emerging, and future genetic technologies, and advising and helping C-SiG Center members properly assess, keep abreast, and implement these often disruptive scientific advances. The C-SiG Genetics and Model Systems Core balances its efforts on: i) Current (RNASeq, mouse ES cells, iPSCs, stable gene delivery and expression with transposons, zebrafish targeted mutants, custom zinc finger nucleases); ii) Expanding (gene knockouts in cells and model organisms and, recently, genome engineering using custom TAL effector nucleases [TALENs]; modeling of disease using custom genome modified zebrafish, fly, mouse and rat model systems); and iii) Near-future (Cas9-based nucleases; in vivo whole animal, single cell non-invasive imaging) genetic approaches. As a central point for access to the latest information on genetic technologies, this core provides an important interface to this dynamic research area through its consultation, educational, and direct, hands-on provision of novel reagents. The C-SiG Genetics and Model Systems Core serves a key role in facilitating access to genetic and animal model system tools for digestive disease-based research projects. The focal Mechanistic Research Themes of the C-SiG grant (Signal Transduction, Ion Channels/Membrane Receptors, and Genetics and Gene Regulation) all benefit from state-of-the-art genetics and animal model system tools, an ever-increasing requirement for modern GI research.

The current objectives of the C-SiG Genetics and Model Systems Core are to:
1) Accelerate research by connecting and educating members to genetics and model systems tools.
2) Deliver new genetics and model systems tools/technologies that are needed by C-SiG members's.
3) Establish cutting-edge genetic tools for genome editing including zinc finger nucleases (ZFNs), TALENs, Cas9 Custom Restriction Enzyme System (CRISPRs) and future locus-specific genome editing tools that can be applied to model organism development including zebrafish, rats, mice and Drosophila.

These objectives are accomplished by:
1) Generating custom reagents including transposon clones, bacterial artificial chromosome (BAC) clones, and TALENs for top-priority projects.
2) Providing education through core-sponsored seminars, Web site, and presentations to C-SiG Member laboratories.
3) Providing consultative services by connecting C-SiG members to genetics tools and institutional infrastructures directly and through a novel online reagent hub.
4) Developing model experimental systems, including zebrafish and genetically manipulated mice, flies and rats, directly and by facilitating internal and external collaborative partnerships.
5) Providing a Web portal hub that is regularly updated by an established genetics staff scientist is a key reference for both GI researchers and Genetics Core staff.
6. Organizing seminars and other, more formal, education and training packages are provided to raise awareness and more generally educate Center members in the potential of these genetics tools.



Services
  • Plasmids, TALENs, transposons, and other DNA constructs support
    • Design
    • Construction
    • Validation
  • Expanded zebrafish technical support
  • Reagents/database
    • Transposons
    • Bacterial artificial chromosomes
    • Custom zinc finger nucleases
  • Consults and training
    • Bioinformatics
      • Microarray design and analysis
      • Next-generation transcriptome and sequence analysis
    • Genetics technologies consults
      • Rodent and zebrafish transgenic/knockout model development support
      • Viral delivery of siRNA
      • Transposons for somatic genetic engineering
      • In vivo imaging


Visit the Core