Marion Bessin Liver Research Center

Core Facilities

The Center has five cores:


SPECIAL ANIMALS CORE

1.Bred Animals

  • Gunn rat (congeneic with the Wistar-RHA strain)
  • Wistar-RHA rat
  • Long Evans Cinnamon (LEC) rat with copper toxicosis
  • Long Evans rat syngeneic to LEC rats (LEA)
  • Dipeptidyl peptidase IV mutant (DPPIV) F344 rat
  • Dipeptidyl peptidase IV knockout in C57BL/6 background
  • NOD-CB17-prkdc-SCID-IL2Rg null mice
  • C57BL/6-Tg(ACTB-RGFP)1Osb/J GFP mice

2.Other Services

    a.Reproduction of Animal Models for Investigators

  • Acute liver injury in rats by treatment with D-galactosamine, CCl4, allyl alcohol, thiocetamide, acetaminophen, monocrotaline, etc.
  • Acute liver injury in mice with azoxymethane, CCl4, concavalin A, monocrotaline, etc.
  • Induction of cirrhosis in rats by chronic administration of CCl4.
  • Radiation-induced liver disease in the rat.
  • Localized and metastatic liver tumors in immunodeficient mice.

    b.Training and/or Assistance with Animal Procedures

  • Partial hepatectomy (68%, 35%) in rats, mice, rabbits and woodchucks.
  • Injections into the peritoneal cavity, portal vein, superior mesenteric vein, spleen, internal jugular vein, femoral vein or tail vein in mouse and rats, and intraorbital injections in mice.
  • Gallbladder or bile duct cannulation and bile collection in rats and mice.
  • Liver perfusion in mice, rats, rabbits, woodchucks and ducks.
  • Intrathymic injections in mice and rats.
  • Intrasplenic, intraportal, subcutaneous cell transplantation.
  • Portal and right atrial pressure measurements.
  • Implantation of osmotic pumps for intraperitoneal, intrasplenic or intravenous infusions.
  • Nuclear medicine methods for quantitating intrahepatic shunting, blood pool analysis with radiolabeled RBC, hepatobiliary excretion of radiolabeled ligands, and biodistribution of radiolabeled liver cells.
  • Analysis of Kupffer cell function with incorporation of carbon particles or radiolabeled technetium sulfur-colloid.
  • Perfusion fixation of liver and other tissues in situ for tissue analysis or electron microscopy.

c.Animal rederivation, breeding or backcrossing
d.Embryo harvest and freezing

Visit the Core