CURE: Digestive Diseases Research Center

Core Facilities


MORPHOLOGY AND IMAGING CORE

Services
  • Immunohistochemistry
  • Assistance and training for Light Microscopy and Digital Photography.
  • Computer assisted image processing and analysis for quantitative determinations of phenotypic changes in gene expression, for morphometric analysis of different cell populations (including measuring areas, or length or surface of villi, counting specific histochemically or neurochemically identified cell populations), and for the quantification of receptors at the plasma membrane and intracytoplasmic compartment
  • Confocal microscopy
  • Ca2+ imaging in conjunction with imaging GFPs and other intracellular fluorescent probes for the analysis of signal transduction pathways in single cells.
  • Fluorescent imaging system for in vitro measurement of intracellular cAMP levels using single excitation dual emission configuration in single cells with attached microinjection system
  • Animal pathology service will provide a detailed anatomical analysis of possible phenotypical or pathological alterations associated to genetic manipulations and a comprehensive and systematic characterization of novel genetically engineered mouse models.
    • tissue procurement and fixation
    • high quality routine tissue processing
    • embedding
    • cutting
    • staining
    • electron microscopy
    • morphometry
    • pathological characterization
  • Non-invasive neuroimaging approaches for investigating the anatomical and biochemical-physiological functions of the brain with high anatomical and temporal resolution as well as to computational infrastructure for archiving imaging
    • Functional MRI
    • PET and SPECT

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