Center for the Study of Inflammatory Bowel Disease, MGH

Core Facilities
The Biomedical Core program has been a central feature of the CSIBD since its inception. These core resources have been developed to accomplish two goals: first, to support the research activities of the center investigators that increase the understanding of IBD, and second, to stimulate new collaborations and interactions. The biomedical cores have been greatly successful in meeting both of these goals. The first goal has been met through both service and educational activities of the individual cores. As a mark of achieving the second goal, the use of these biomedical cores has led to projects that would not have been undertaken if the cores had not been available. We are continually working to maintain this high level of research support and investigator interaction. The Center has Five cores:


GENETICS, GENOMICS AND MOLECULAR BIOLOGY CORE

The major roles of the Genetics, Genomics and Molecular Biology (GGMB) Core are 1) to provide services in the utilization of advanced genetics and molecular biological techniques and 2) to facilitate the implementation of advances in genomics and molecular biology to IBD research. Application of genetics and molecular biological approaches is central to the evaluation of processes underlying the development of IBD according to the hypotheses that serve as a foundation for this center. This core principally provides access to high-demand, mission-enabling tools for the investigator cohort participating in the CSIBD. The core also provides access to capital or resource-intensive methodologies, such as laboratory automation cDNA and siRNA libraries, and BAC-centered recombinant DNA techniques.
The importance of molecular methods for the understanding of mechanisms of immunity and generating transgenic/knockout mice is undeniable, and their impact and dissemination throughout biological disciplines grow every year. These techniques permit the identification and characterization of genes regulating epithelial and immune cell function, analysis of the expression of these genes, determination of the functions and interactions of the encoded proteins, and expression of reporter genes and proteins to allow cellular localization and physiological analysis. For those laboratories with limited expertise in molecular biology, the core has developed a major educational effort consisting of the annual current techniques course in molecular genetics and training in genetic epidemiology, quantitative biology, and genomic medicine. Learn More...

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